Fluorescence Measurement Services
Bispectral fluorescence, also know as bispectral fluorescence colorimetry,
is a method of determining both the luminous and reflected component of materials
that exhibit fluorescence. This technique requires a double monochromator system,
with both monochromatic illumination and a monochromator for the reflected/emitted
light to separate the fluorescent and reflected components on the output side.
This technique is the only way to fully characterize materials such as high
visibility safety materials ("daylight fluorescents") and for the
optical brighteners used in the textile and paper industries.
Avian Technologies is the only laboratory in the world to offer
bispectral fluorescence measurements using directional/directional
(45/0) geometry.
Our bispectral instrument is:
A Labsphere BFC-450 Bispectral Fluorescence Colorimeter. This
instrument, which won a Photonics Spectra "Product of the
Year" award, was the first commercial instrument to measure
bispectral fluorescence. The instrument illuminates the sample
with a circumferential beam at 45 degree incidence and collects
at normal. The geometry of the instrument meets CIE 15.2 optical
specifications for a 45/0 instrument.
Measurements can be provided that give full bispectral data for total
spectral radiance and the radiance of the reflected and fluorescent components,
colorimetric data, along with ISO brightness.
For further information and an introduction to bispectral fluorescence,
see A. Springsteen"An Introduction to the Measurement of
Color of Fluorescent Materials", Analytical Chimica Acta,
Vol. 380 (1999) and related articles in the same volume. See also:
slp.nist.gov/appearance/springsteen.pdf
and J. Leland, N. Johnson and A. Arecchi "Principles of Bispectral
Fluorescence Colorimetry" in A.V. Arecchi (Ed.) Photometric
Engineering of Sources and Systems, 29-30 July 1997, 3140, 76-87,
San Diego, USA.
Excitation/Emission Matrix Fluorescence
Through Avian Technologies partnership with PhytoPhotonics,
we now have the ability to measure excitation/emission matrix
spectra of luminescent materials.
(instrument details here)
Typical data presentation is shown below as 3D plot or 2D contour.
The geometry of measurement is at 45:0. The image below shows the illumination of a sample mounted in the instrument.
|
