Fluorescence Measurement Services

Questions? Contact Art Springsteen: Email or (603)526-2420
Quotation? Contact Kathy Springsteen: Email or (603)526-2420

Bispectral fluorescence, also know as bispectral fluorescence colorimetry, is a method of determining both the luminous and reflected component of materials that exhibit fluorescence. This technique requires a double monochromator system, with both monochromatic illumination and a monochromator for the reflected/emitted light to separate the fluorescent and reflected components on the output side. This technique is the only way to fully characterize materials such as high visibility safety materials ("daylight fluorescents") and for the optical brighteners used in the textile and paper industries.

Our measurement capabilities for complete bispectral colorimetry are currently in flux. As such these measurements are only available as custom services and not traceable. Given those limitations, we are still able to provide full bispectral data for total spectral radiance and the radiance of the reflected and fluorescent components, colorimetric data, along with ISO brightness.

For further information and an introduction to bispectral fluorescence, see A. Springsteen "An Introduction to the Measurement of Color of Fluorescent Materials", Analytical Chimica Acta, Vol. 380 (1999) and related articles in the same volume. See also: J. Leland, N. Johnson and A. Arecchi "Principles of Bispectral Fluorescence Colorimetry" in A.V. Arecchi (Ed.) Photometric Engineering of Sources and Systems, 29-30 July 1997, 3140, 76-87, San Diego, USA.

Excitation/Emission Matrix Fluorescence

Through Avian Technologies partnership with PhytoPhotonics, we now have the ability to measure excitation/emission matrix spectra of luminescent materials. (instrument details here) Typical data presentation is shown below as 3D plot or 2D contour.

The geometry of measurement is at 45:0. The image below shows the illumination of a sample mounted in the instrument.